Coding

Part:BBa_K4800025:Design

Designed by: Yao Yao   Group: iGEM23_NJTech-China-B   (2023-10-08)


Hsd36


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 37
    Illegal AgeI site found at 109
    Illegal AgeI site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

Hsd36 is from Oenococcus alcoholitolerans.

References